The ethanol extract of seed of Achyranthes aspera was investigated for antimicrobial activity at10 mg/ml concentrations by using Kirby-Bauer disc diffusion method against gram positive and gram negative organisms like Escherichia coli, Staphylococcus aureus, Streptococcus pyogenes, Pseudomonas aeruginosa. After incubation for 24 hrs, the zone of inhibition was compared with standard antibiotic genatmycin (10 μg/ disc). From the dose dependent study it was observed that the ethanol seed extract was more potent than leaf extract. Flavonoids and tannins present in the both ethanol and aqueous extract may be responsible for the antimicrobial activity. The minimal inhibitory concentrations (MICs) of ethanol extract of seeds were determined by broth dilution technique which ranged from 500 μg to >800 μg /ml and 700 μg to >1000 μg /ml respectively, indicating that A.aspera seed extract was highly effective against E.coli with lower MIC value. There were some biochemical alterations induced by A.aspera. These results indicate that A.aspera fruit possesses potential broad spectrum antimicrobial activity.
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